1. Principle and characteristics of solid phase extraction device
principle
Solid-phase extraction (Solid-Phase Extraction) for short is a widely used and popular sample preparation technology, which is to use a solid adsorbent to adsorb the target compound in the sample and separate it from the sample matrix and interfering compounds. Then elute with eluent or desorption by heating to achieve the purpose of separating and enriching the target compound. It is based on the traditional liquid-liquid extraction (LLE) based on the principle of similar miscibility between similar substances and combined with the widely used basic knowledge of liquid chromatography (HPLC) and gas chromatography (GC) stationary phases.
In the solid phase extraction process, the attractive force of the solid relative to the analyte is greater than that of the sample mother liquid. When the sample passes through the SPE column, the analyte is adsorbed on the solid surface, and other components pass through the column with the sample mother liquid. It eluted. SPE itself can be divided into four types according to its principle: reverse SPE (RP-SPE), forward (NP-SPE), ion exchange SPE (IE-SPE), and adsorption SPE (Absorption). In most cases, solid phase extraction is used to process liquid samples to extract, concentrate, and purify volatile and semi-volatile compounds in the sample; when it is needed for solid samples, the solid samples must be processed into liquid state first.
When the number of individual SPE treatments increases, the timeliness of the analyst becomes frequent and complicated. Human errors greatly affect the reproducibility of the processed samples, which is not conducive to the quantitative and qualitative work of the analysis results. The company introduced technical support imported from the United States, SPE multi-tube vacuum solid phase extraction device (Vacuum Manifold) came into being, the company's HP-6019 solid phase extraction device can batch process multiple SPE work items at the same time, and will not cross-infect each other. The closed and integrated anti-fogging observation chamber makes your SPE work progress clear and clear.
Instrument installation and debugging steps
â‘´ Carefully take out the solid phase extraction device and put it gently on the workbench.
⑵ Carefully remove the upper cover plate of the SPE device (handle gently to avoid damage to the small tube), insert the standard test tube into the hole of the partition in the vacuum chamber, then cover the upper dry cover plate and ensure that the guide tube under the cover plate It has a good one-to-one correspondence with the test tubes, and the square sealing ring of the cover plate has a good seal with the vacuum chamber. If it is not easy to seal, you can tighten it with a rubber band to increase the tightness.
(3) If you purchase an independent adjustment, you must first insert the adjustment valve into the extraction hole of the cover;
â‘· If you don't need to make 12 or 24 samples at a time, insert the unused extraction hole into the needle tight valve;
⑸ If you choose an independent regulating valve, turn the regulating valve knob of the unused extraction hole to a horizontal sealing state;
⑹ Insert the solid phase extraction cartridge into the extraction hole of the upper cover or the valve hole (turn the adjustment valve knob to the upright open state); connect the extraction device and the vacuum pump with a rubber tube, and tighten the pressure adjustment valve;
⑺Inject the samples or reagents to be extracted into the extraction column separately, start the vacuum pump, then the samples in the extraction column will flow through the extraction column to the following test tube under the effect of negative pressure. At this time, the liquid flow rate can be adjusted and controlled by adjusting the pressure reducing valve.
(8) After the liquid in the needle tube is pumped off, turn off the vacuum pump, remove the enrichment column from the device, remove the cover plate of the device, take out the test tube and discard it.
⑼ If you don't want to use the test tube to pick up the liquid, you can take out the test tube rack, put it into a container of appropriate size, take it out after the first extraction and discard it.
⑽ Put the clean test tube into the device, cover the upper cover, insert the SPE cartridge, add the needle with the required extraction solvent, start the vacuum pump, turn off the power after the liquid is drained, remove the test tube and use it. The extraction and sample preparation are completed.
⑾ Place the test tube in a nitrogen blow-dryer and purge and concentrate with nitrogen. The preparation is completed.
â‘¿ Dispose of the solvent in the test tube, and rinse the test tube for reuse.
â’€ In order to save the use cost of SPE column, after each use, the SPE column should be rinsed with eluent to ensure the activity of its packing.
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