Abstract: The processing raw materials of animal-derived feed are all derived from the animals in nature or some tissues or organs of themselves. Generally speaking, these tissues or organs are wastes generated during animal slaughter.
Near-infrared spectroscopy (NIRS) is one of the most widely used technologies in the feed industry, and it can be used for the detection of animal-derived components in feed. The principle of NIRS is that molecular substances in feed can absorb light of different wavelengths. Its advantages are rapid detection, no harmful reagents, less sample volume required, non-destructive detection, good economic repeatability and the potential to be developed as a commercial instrument. The main disadvantage is that this technique is an indirect detection, so the reference value of a large number of samples is needed to form a calibration and reference model.
At present, many countries in the world, especially the EU countries, have formulated various laws to ensure the biological safety of feed in order to prevent mad cow disease, mainly to control the application of animal-derived ingredients in feed, and prohibit the addition of meat and bone meal in ruminant feed And the prohibition of intra-species circulation of animal-derived ingredients is the central measure to prevent mad cow disease. The effective implementation of these laws and regulations needs to be backed up by effective methods that can detect animal-derived ingredients in feed.
The ideal analysis method for the identification of meat and bone meal should be specific, sensitive, fast, affordable and high-throughput. Due to different sources of materials, no single method can meet the above requirements. The choice of method depends on many factors, such as the purpose of the test, the nature of the sample, the size and number of samples, specificity and sensitivity requirements, time period, cost, acceptable facilities and equipment, etc. One or more methods should be selected as the detection method according to the specific conditions of the sample being tested. Through the evaluation of the advantages and disadvantages of various methods, it can be seen that the relationship between the various methods is complementary rather than competitive. For example, the near-infrared method and the immunological method are used as the screening method, and the microscopy method and the PCR method have high legal value; in addition, the near-infrared method is not affected by the heat treatment, and can be complementary to the PCR method and the immunological method. The continuous improvement of various methods at present will eventually make the reference laboratory point out a suitable method for specific purpose analysis. This will be a method system composed of a series of different methods.
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