Shanghai Haozhuang Instrument Co., Ltd. develops the LNB brand anaerobic incubator, which is energy-saving and environmentally friendly, leading the country. Professional manufacturing is more secure and affordable
1. The anaerobic incubator should be placed in a place where the temperature difference is small and the operation is convenient. The direct sunlight and the heating equipment should be avoided, and it should be placed steadily; 2. The mixed gas bottle and nitrogen bottle should be placed stably, and the pressure relief valves should be installed separately. (Including pressure gauge), placed in an appropriate position; 3. Connect the gas path and check the gas path, in order to prevent air leakage, if necessary, can be bonded with sealant at each nozzle; Use (1) The anaerobic environment of the operation room is formed : 1. Place the necessary accessories and appliances according to the requirements of use, and put two non-toxic plastic bags into the operating room; 2. Turn on the lights with the power supply, turn on the temperature controller, adjust the required temperature and safe temperature; 3. Put 1000g of palladium pellets (closed) and 500g of desiccant in the operation room, and put the Meilan indicator (closed); 4. Close the door of the sampling room and draw a vacuum to verify; 5. The first replacement in the operation room (nitrogen) Replacement): A. First insert the rubber tube into the air inlet of the operating room, and insert the other end into the plastic bag; B. Connect the nitrogen gas inlet, open the nitrogen control valve 1, make the two plastic bags full of nitrogen, close the valve 1, and then Tighten the mouth of the bag; C. Put the latex gloves on the flange ring of the observation board and tighten; D. Put the plastic Nitrogen in the bag is gradually placed in the operating room until all is released. 6. The second replacement of the operation room (nitrogen replacement) repeats the nitrogen filling process. The sampling room is evacuated first, and pay attention to open and close the exhaust with the foot switch at any time. 7. The third replacement of the operating room (mixed gas replacement): (mixed gas ratio is N2 85%, H2 10%, CO2 5%) A. Change the gas path and open the mixed gas valve 3 for intake. Vacuum, and use the foot switch to open and close the exhaust at any time; B. After the mixed gas is filled in the plastic bag, close the mixed gas valve, and open the valve 5, let the mixed gas pass the flow meter, adjust the flow meter, the flow rate is 10ml per minute Left and right; C. The mixed gas in the plastic bag is gradually discharged into the operation room; D. After three times of ventilation, the oxygen content of the gas in the operation room is already in a very trace state. 8. Turn on the grain deaerator in the operation room, connect the power of the deaeration catalyst for catalytic deaeration, and open the Meilan indicator (Meilan ampoules) to observe its discoloration after one hour. Environment; 9. Turn on the ultraviolet sterilization lamp and sterilize the room. The sterilization time is determined according to the specific experiment. (2) Placement and cultivation of strains: 1. Check the door of the sampling chamber and close it tightly; 2. Open the door of the sampling chamber and close the outside door after placing the strains in the sampling chamber; 3. Three times of nitrogen replacement in the sampling chamber ： Turn on the vacuum pump, first stop the vacuum above 500ml Hg (66kPa), then manually open the nitrogen valve 2 inlet, return the pointer to zero position, and close the valve 2. Repeat the operation once for the second time. During the third operation, stop the vacuum above 500ml Hg (66kPa), then open the valve 4 to enter the mixed gas, return the pointer to the zero position, and then drop the valve 4. The three times of nitrogen filling and replacement process in the sampling chamber is ended; 4. If the selected vacuum degree is low, the number of replacements needs to be increased; 5. Open and close the doors inside and outside the sampling chamber, and then draw a low vacuum of 100ml Hg (13kPa) for inspection; 6. Conditions for long-term continuous use of anaerobic incubators: A. Open the Meilan indicator in the operating room every day to observe, if it is abnormal, you must re-ventilate; B. To continuously input a small amount of mixed gas for a long time, so that the supplemental nitrogen can Combined with a trace amount of oxygen through catalytic absorption to ensure indoor anaerobic state, the flow rate of the supplemental gas mixture is selected to be about 10ml per minute; C. Continuous culture operation for one day, replacing the deaerator and desiccant once. 7. The temperature of the cultivation room in the operation room can be arbitrarily selected and controlled. 8. Adjust the output pressure of the mixed gas cylinder and nitrogen cylinder: adjust the pressure reducing valve to make the output pressure about 0.1Mpa.
Haozhuang R & D team's forward-looking technology development vision and strong technical strength are actively in line with the needs of scientific research. By promoting the continuous innovation of Haozhuang brand, Haozhuang's differentiated competitive advantage has been continuously improved, making Haozhuang always proud. The status of the industry leader. Taking advantage of the opportunity of the industrial gradient transfer in Shanghai where Haozhuang Company is located, Haozhuang will implement its headquarters to enter Hong Kong in the next five years, and will deploy Haozhuang Hong Kong Company to the global industry.
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